recombinant human αvβ6 integrin Search Results


recombinant human integrin αvβ6/itgav&itgb6 heterodimer protein  (ACROBiosystems)
90
ACROBiosystems recombinant human integrin αvβ6/itgav&itgb6 heterodimer protein
Self-assembly, and nano fibrillar transformation of smart peptide monomer GRGDLGRL-KLVFF-GGK-PheoA (P1). Fluorescent intensity (a) and absorbance (b) of NPs1 solutions with different water content (including 0%, 20%, 40%, 60%, 80%, 99.5%). The excitation wavelength was 405 nm. (c) TEM images of fresh NPs1 at the water and DMSO ratio of 995:5. Fluorescent intensity (d) and variation in size distribution (e) of NFs1 (NPs1 incubated with <t>integrin</t> <t>αvβ6</t> protein at the molar ratio of 500:1) within 48 h. (f) TEM image of NFs1 transformed by initial NPs1 after incubation with αvβ6 protein for 24 h. The concentration of NPs1 related to these experiments was 20 µM, and all these experiments were repeated three times.
Recombinant Human Integrin αvβ6/Itgav&Itgb6 Heterodimer Protein, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Self-assembly, and nano fibrillar transformation of smart peptide monomer GRGDLGRL-KLVFF-GGK-PheoA (P1). Fluorescent intensity (a) and absorbance (b) of NPs1 solutions with different water content (including 0%, 20%, 40%, 60%, 80%, 99.5%). The excitation wavelength was 405 nm. (c) TEM images of fresh NPs1 at the water and DMSO ratio of 995:5. Fluorescent intensity (d) and variation in size distribution (e) of NFs1 (NPs1 incubated with integrin αvβ6 protein at the molar ratio of 500:1) within 48 h. (f) TEM image of NFs1 transformed by initial NPs1 after incubation with αvβ6 protein for 24 h. The concentration of NPs1 related to these experiments was 20 µM, and all these experiments were repeated three times.

Journal: Drug Delivery

Article Title: Phototherapeutic effect of transformable peptides containing pheophorbide a on colorectal cancer

doi: 10.1080/10717544.2022.2075987

Figure Lengend Snippet: Self-assembly, and nano fibrillar transformation of smart peptide monomer GRGDLGRL-KLVFF-GGK-PheoA (P1). Fluorescent intensity (a) and absorbance (b) of NPs1 solutions with different water content (including 0%, 20%, 40%, 60%, 80%, 99.5%). The excitation wavelength was 405 nm. (c) TEM images of fresh NPs1 at the water and DMSO ratio of 995:5. Fluorescent intensity (d) and variation in size distribution (e) of NFs1 (NPs1 incubated with integrin αvβ6 protein at the molar ratio of 500:1) within 48 h. (f) TEM image of NFs1 transformed by initial NPs1 after incubation with αvβ6 protein for 24 h. The concentration of NPs1 related to these experiments was 20 µM, and all these experiments were repeated three times.

Article Snippet: To prepare the nanofibrils (NFs), nanoparticles (20 μM) were cultured with the integrin αvβ6 protein (recombinant human integrin αvβ6/ITGAV&ITGB6 heterodimer protein, ACROBiosystems) for 24 h at the molar ratio of peptide/protein 500:1 (the same as the following experiment).

Techniques: Transformation Assay, Incubation, Concentration Assay

Transformation and retention of NPs on the cancer cells' surface. (a) Fluorescence distribution images of integrin αvβ6 protein and different NPs (incubation with cells for 8 h) on HT-29 tumor cells. Integrin αvβ6 protein: Green color (excitation wavelength = 488 nm). PheoA: Red color (excitation wavelength = 405 nm). (b) Fluorescence distribution images of cancer cells treated with NPs1 for 1, 8, and 24 h. (c) SEM images of tumor cells incubation with different NPs for 24 h. (d) Fluorescence signal retention on tumor cells. Cells were incubated with different NPs for 8 h, respectively, then the complete DMEM was removed and replaced with flesh complete DMEM without NPs for another 16 and 40 h. The concentration of NPs related to these experiments was 20 µM, and all these experiments were repeated three times.

Journal: Drug Delivery

Article Title: Phototherapeutic effect of transformable peptides containing pheophorbide a on colorectal cancer

doi: 10.1080/10717544.2022.2075987

Figure Lengend Snippet: Transformation and retention of NPs on the cancer cells' surface. (a) Fluorescence distribution images of integrin αvβ6 protein and different NPs (incubation with cells for 8 h) on HT-29 tumor cells. Integrin αvβ6 protein: Green color (excitation wavelength = 488 nm). PheoA: Red color (excitation wavelength = 405 nm). (b) Fluorescence distribution images of cancer cells treated with NPs1 for 1, 8, and 24 h. (c) SEM images of tumor cells incubation with different NPs for 24 h. (d) Fluorescence signal retention on tumor cells. Cells were incubated with different NPs for 8 h, respectively, then the complete DMEM was removed and replaced with flesh complete DMEM without NPs for another 16 and 40 h. The concentration of NPs related to these experiments was 20 µM, and all these experiments were repeated three times.

Article Snippet: To prepare the nanofibrils (NFs), nanoparticles (20 μM) were cultured with the integrin αvβ6 protein (recombinant human integrin αvβ6/ITGAV&ITGB6 heterodimer protein, ACROBiosystems) for 24 h at the molar ratio of peptide/protein 500:1 (the same as the following experiment).

Techniques: Transformation Assay, Fluorescence, Incubation, Concentration Assay

The therapeutic effect of different NPs in vitro . (a) Temperature variation of NPs1 and NFs1 (NPs1 incubation with αvβ6 protein for 24 h) during four irradiation-cooling cycles (laser irradiation for 120 s, and then cooling for 90 s). The concentration of NPs1 and NFs1 was 50 μM. (b) ROS generation of different NPs (20 μM) with/without αvβ6 protein by using DCFH fluorescence probe after irradiation for 30 s (excitation wavelength = 488 nm). (c) Cell viability of HT-29 cancer cells after incubation with different NPs for 8 h, with irradiation (30 s) or not. The fluorescence images (Green color: apoptosis cells; Red color: necrosis cells) (d) and quantitative analysis (e) of apoptosis cells after treatment with different NPs (20 μM) for 8 h, then irradiation for 30 s or not. The excitation wavelengths of FITC and PI were 488 nm and 535 nm, respectively. Cellular ROS generation via flow cytometry (f) and relative quantitative analysis (g). DCFH-DA Cellular Reactive Oxygen Assay Kit was used to detect ROS after treating cancer cells with different NPs for 8 h with/without irradiation for 30 s (excitation wavelength = 488 nm). (h) Cell viability of long-term retention of NPs. Treating cancer cells with different NPs for 8 h, and replaced with a new flesh medium for 16 h prior to irradiation for 30 s, then the viability of the cells were assayed by MTT. (i) Apoptosis and ROS generation of long-term retention of NPs. Before irradiation for 30 s, cancer cells were treated with different NPs for 8 h plus another 16 h with fresh medium. Using an inversion microscope to detect apoptosis, and flow cytometry to detect ROS generation. The irradiation powder density remained at 0.4 W/cm 2 in these experiments. Data were presented as mean ± s.d. ( n = 3, *P < .1, **P < .01, ***P < .001).

Journal: Drug Delivery

Article Title: Phototherapeutic effect of transformable peptides containing pheophorbide a on colorectal cancer

doi: 10.1080/10717544.2022.2075987

Figure Lengend Snippet: The therapeutic effect of different NPs in vitro . (a) Temperature variation of NPs1 and NFs1 (NPs1 incubation with αvβ6 protein for 24 h) during four irradiation-cooling cycles (laser irradiation for 120 s, and then cooling for 90 s). The concentration of NPs1 and NFs1 was 50 μM. (b) ROS generation of different NPs (20 μM) with/without αvβ6 protein by using DCFH fluorescence probe after irradiation for 30 s (excitation wavelength = 488 nm). (c) Cell viability of HT-29 cancer cells after incubation with different NPs for 8 h, with irradiation (30 s) or not. The fluorescence images (Green color: apoptosis cells; Red color: necrosis cells) (d) and quantitative analysis (e) of apoptosis cells after treatment with different NPs (20 μM) for 8 h, then irradiation for 30 s or not. The excitation wavelengths of FITC and PI were 488 nm and 535 nm, respectively. Cellular ROS generation via flow cytometry (f) and relative quantitative analysis (g). DCFH-DA Cellular Reactive Oxygen Assay Kit was used to detect ROS after treating cancer cells with different NPs for 8 h with/without irradiation for 30 s (excitation wavelength = 488 nm). (h) Cell viability of long-term retention of NPs. Treating cancer cells with different NPs for 8 h, and replaced with a new flesh medium for 16 h prior to irradiation for 30 s, then the viability of the cells were assayed by MTT. (i) Apoptosis and ROS generation of long-term retention of NPs. Before irradiation for 30 s, cancer cells were treated with different NPs for 8 h plus another 16 h with fresh medium. Using an inversion microscope to detect apoptosis, and flow cytometry to detect ROS generation. The irradiation powder density remained at 0.4 W/cm 2 in these experiments. Data were presented as mean ± s.d. ( n = 3, *P < .1, **P < .01, ***P < .001).

Article Snippet: To prepare the nanofibrils (NFs), nanoparticles (20 μM) were cultured with the integrin αvβ6 protein (recombinant human integrin αvβ6/ITGAV&ITGB6 heterodimer protein, ACROBiosystems) for 24 h at the molar ratio of peptide/protein 500:1 (the same as the following experiment).

Techniques: In Vitro, Incubation, Irradiation, Concentration Assay, Fluorescence, Flow Cytometry, Microscopy